Application of fluorescence in situ hybridization and karyotype analysis in diagnosis of typical and atypical chronic myeloid leukemia

Objective To investigate the application value of the fluorescence on situ hybridization and karyotype analysis in differential diagnosis of chronic myelogenous leukemia and atypical chronic myeloid leukemia. Methods A retrospectively analysis of karyotype and FISH test were performed in 78 cases of CML and 9 cases of aCML. Results The results showed that nine cases had no performed karyotyping analysis among 78 confirmed CML cases,however,it was not confirmed. In the remaining 69 cases,4 cases had normal karyotyping analysis,65 cases were Philadelphia positive,6 cases had other abnormal chromosome. All of CML patients were detected in BCR/ABL fusion gene abnormalities. It was Philadelphia and FISH negative in 9 cases of aCML patients,meanwhile six cases had other abnormal chromosome. Conclusions Potential CML patients should do karyotyping and FISH detection simultaneously,avoiding the CML being misdiagnosed as aCML,at the same time,complex karyotype can be found. It has importance significance in the prognosis. Key words: Chronic myelogenous leukemia; Atypical chronic myelogenous leukemia; Fluorescence in situ hybridization; Karyotype analysis