Different diagnostic modalities for the study of Kumari (Aloe vera (l.) burm.) swarasa (Juice) Bhavita Trivanga Bhasma with respect to baseline microbial profile

Background: In Ayurveda, metal-based preparations, that is Bhasma (incinerated Rasaushadhi), are indicated for the treatment of several diseases. Trivanga Bhasma is a classical Ayurvedic preparation containing Bhasmas of three metals, namely Vanga (Tin), Naga (Lead), and Yashada (zinc), and it is indicated in Madhumeha (diabetes) and MutraRoga (urinary disorders), Napunasakta (impotency), Vandhyatva (Infertility), Swetapradara (Leucorrhea), Vata-Pittadosa, and as Shaktivardhaka(strength). Aims: The study was carried out the stability of Kumari Swarasa Bhavita Trivanga Bhasma concerning its microbial profile. Materials and Methods: A sample of Kumari Swarasa Bhavita Trivanga Bhasma was prepared and studied to check microbial contamination at regular intervals. Results: Every time, the sample was subjected to the microbiological study from the date of the preparation to the date of the last microbiological study. No contamination was found in the microbiological study. Discussion: Hence, the present study was carried out to observe the stability study of Kumari Swarasa Bhavita Trivanga Bhasma concerning microbial contamination of the sample prepared and preserved in different climatic and temperature conditions. Thus, a baseline microbial profile was studied at regular intervals. At the end of the study, it was found that the sample was not shown the presence of any microbes. Conclusion: In the microbiological study of Kumari Swarasa Bhavita Trivanga Bhasma, there was no growth found of microorganisms (bacterial or fungal), from the date of preparation, shown shelf life for 296 days. Hence, in the present study, the stability test of Kumari Swarasa Bhavita Trivanga Bhasma concerning microbiological findings was negative at room temperature, warm and cold, dry, and humid conditions.