Dynamic analysis of the lymphocyte subsets in HCV children with different genotypes during treatment

Objective To discuss the changes of lymphocyte subsets in HCV children with different genotypes during treatment with pegylated interferon alfa-2b and ribavirin.Methods The genotype of 45 HCV infected children were identified by real time PCR.The lymphocyte subsets were dynamically detected by BD FACSCalibur flow cytometer with four color MultiTEST IMK Kit during the treatment.Results For the children with 1 b genotype,after 24 weeks,the CD4 +T cells were higher than pre-treatment(P ＜ 0.05).For the children with 2a genotype,after 12 weeks and after 24 weeks,the CD3 +T cells and CD4+T cells significantly increased while the NK cells decreased than pre-treatment (P ＜ 0.05).Conclusions The lymphocyte subsets of HCV children with 2a genotype were different from 1 b genotype during trentment with pegylated interferon alfa-2b and ribavirin. Key words: Hepatitis C ;  Genotype;  Child, preschool;  Lymphocyte subsets