Correlation Between The Level of Lp-PLA2, MDA, F2-Isp in Serum And Aortic Tissue With The Number of Foam Cells at Atherogenesis Process in Wistar Rats

Background: Atherogenesiswas initiated by cholesterol deposits on foam cell in sub intimae of blood vessel stress oxidation. Atherogenesis in non-hypercolesterolemiausually undergoes an increased level of Lp-PLA2. It, therefore, needs to evaluate the role of Lp-PLA2in the foam cell formation. Aims: To explain the role of Lp-PLA2 in the foam cell forming, to correlate the level of Lp-PLA2 ,MDA, F2-Isp in aorta with foam cell number (FCs) as well as to correlate the level of Lp-PLA2 , MDA, F2-Isp contents in serum with their contents in aorta and the correlation with FCs. Methods. 30 rats aged 2 months, with their weight averaging from 150-200g, were divided into the control group and the treatment one where the latter was fed hyperlidemia for 2,8 and 12 weeks. The measurement level of LDL-C, MDA, F2-isp and Lp-PLA2in serum was performedas well as aorta and FCs. Data was analysed using anova, t-Test, path analysis and correlation. Results: Research result indicated that: (1) The level of MDA(a), F2-Isp(s) and Lp-PLA2(s) positively correlated with FCs, Lp-PLA(s)having the highest correlation value. (2) Lp-PLA2(a),MDA(s) and F2-Isp(s,a)did not correlate with FCs. (3) There was a positive correlation between Lp-PLA2 with MDA and F2-Isp in both serum and aorta. Conclusion: The enzyme of Lp-PLA2 acts as an activator in forming the foam cell with stimulated stress oxidation.