The Effect of Residual Food Stain on Candida Albicans Colonisation of Denture Acrylics
2020
Objectives: In the UK, 19% of adults wear dentures. Failure to keep a denture clean can lead to staining
from foods, along with subsequent colonisation of the denture and associated mucosa by microorganisms,
particularly Candida albicans. This colonisation can potentially lead to chronic erythematous candidosis
and other oral infections. This study investigated the association between staining of denture acrylics by
different food types and subsequent C. albicans colonisation.
Materials and Methods: Chemically polymerised acrylic specimens were produced and stained for 14 days
with six different combinations of food stains. The level of acrylic staining was determined
spectrophotometrically. Specimens were then incubated in Sabouraud-dextrose broth (SAB) or SAB
inoculated with Candida albicans. Confocal laser scanning microscopy coupled with propidium iodide
staining of C. albicans was used to determine the extent of C. albicans colonisation to these acrylics. Results
analysed descriptively and by one-way analysis of variance (ANOVA), one sample student t-test, and
Dunnett’s test.
Results: Acrylics in Group 4 (stained with spices, tomato puree, acai berry juice and sunflower oil)
exhibited highest staining but had low C. albicans colonisation. Highest C. albicans colonisation occurred
with Group 5 (sunflower oil) stained acrylics. The unstained control acrylic group had lowest colonisation.
Conclusion: This study demonstrated that staining acrylics with certain foods promoted C. albicans
colonisation, but this was not associated with level of visual staining. Further research is required to
determine the precise mechanism(s) by which residual food stains promote candidal colonisation on denture
acrylics. This knowledge may then be used by dental professionals to advise patients on improving denture
hygiene to improve not only denture aesthetics but also minimise Candida biofilms.
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