Cloning and sequence analysis of VP2 gene and NS1 gene of blue fox parvovirus

Two pairs of primers were designed based on the VP2 gene and NS1 gene of the strains of canine parvovirus and feline parvovirus according to GenBank.Using blue fox parvovirus Tai'an isolate(BFPV-TA)as template,the VP2 gene and NS1 gene were amplified with PCR,which were cloned into pMD18-T vector,sequenced and analyzed.As a result,the ORF of the VP2 gene of BFPV-TA was 1 755 bp and encoded 584 amino acids.The VP2 protein possessed three substitutions,219I→V,300G→P and 411E→A.The ORF of the NS1 gene of BFPV-TA was 2 007 bp and encoded 668 amino acids.The NS1 protein had eight substitutions,43R→H,135H→Y,172K→R,179A→E,350D→N,409I→V,574I→V,616D→V.The nucleotide homologies of VP2 genes between BFPV-TA and the CPV,FPV references are from 98.4% to 99.4%,the identity of NS1 from 98.6% to 99.1%.VP2 gene phylogenetic analysis shoued that CDVFOX-TA and the CDV wild strains had the same ancestor.BFPV-TA and FPV had the nearer inherit distance,but the clear cluster was observed based on NS1 gene phylogenetic analysis.