Absence of large intragenic rearrangements in the DPYD gene in a large cohort of colorectal cancer patients treated with 5‐FU‐based chemotherapy
2010
WHAT IS ALREADY KNOWN ABOUT THIS SUBJECT
• Dihydropyrimidine dehydrogenase (DPD) is the enzyme responsible for the elimination of approximately 80% of the administered dose of 5-fluorouracil (5-FU).
• Mutations in the DPD-coding gene have been shown to increase the risk of severe toxicity in 5-FU treated patients.
• The IVS14+1G>A is the most common DPYD mutation.
WHAT THIS STUDY ADDS
• The intragenic rearrangements of DPYD using multiplex ligation-dependent probe amplification (MLPA) were studied for the first time in a large series of 234 colorectal cancer patients treated with 5-FU-containing chemotherapy.
• No deletions or duplications of one or more DPYD exons were detected. The presence of the IVS14+1G>A mutation was also excluded.
• These data show that neither the large genomic rearrangements in the DPYD gene nor the IVS14+1G>A mutation are responsible for the serious toxicity associated with a 5-FU containing regimen in this cohort of Spanish patients.
AIMS
To study the relationship between the toxicity associated with a 5-FU-based therapy and the presence of (i) the large intragenic rearrangements in the DPYD gene and (ii) the IVS14+1G>A mutation.
METHODS
We used the multiplex ligation-dependent probe amplification technique (MLPA) to study genomic DNA from 234 colorectal cancer patients treated with 5-FU-based chemotherapy.
RESULTS
We did not detect any deletion/duplication in the DPYD gene. The presence of the IVS14+1G>A mutation was also excluded.
CONCLUSIONS
Neither the large genomic rearrangements in the DPYD gene nor the IVS14+1G>A mutation play a significant role in the development of serious toxicity associated with a 5-FU containing regimen.
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