WITHDRAWN: WSTF Phosphorylation Specifically Links H3K9ac with H4K16ac through PCAF/WSTF/MOF Complex.

Abstract WSTF was identified in nearly all patients with Williams Syndrome. It is unknown whether this multifunctional atypical kinase is involved in other human diseases. In this work, we confirm a WSTF phosphorylation dependent connection between WSTF, PCAF and MOF in human tumors. We reveal functional and physical interplays between WSTF and these two histone acetyltransferases. The MOF/WSTF/PCAF complex can synchronously regulate H3K9ac and H4K16ac in different patterns. We establish that the association of PCAF and WSTF was increased when WSTF was phosphorylated, whereas the association of MOF and WSTF was reduced. Further, we identify the connection with WSTF regulates the activities of histone acetyltransferases of MOF and PCAF. Moreover, WSTF targets the transcription of tumor-associated genes and inhibiting the phosphorylation of WSTF counteracts proliferation and migration of tumor cells. Together, our findings support an unexpected link between WSTF and the specific cooperation between H3K9ac and H4K16ac.