Quinolone-Resistant Haemophilus influenzae in a Long-Term Care Facility: Clinical and Molecular Epidemiology

We describe a clonal outbreak of quinolone-resistant Haemophilus influenzae (QRHI) from an affiliated long-term care facility (LTCF-A); the outbreak was associated with the clinical use of levofloxacin, which was determined to be a risk factor for acquisition of QRHI. The minimum inhibitory concentration to which 90% of isolates were susceptible (MIC 90 ), as determined by broth microdilution, was >4 µg/mL for levofloxacin, >2 µg/mL for moxifloxacin, >2 µg/mL for gatifloxacin, and 8 µg/mL for gemifloxacin. The MIC 90 , as determined by Etest (AB Biodisk), was >32 µg/mL for levofloxacin, ciprofloxacin, moxifloxacin, and gatifloxacin. Having been a resident at LTCF-A and having chronic obstructive pulmonary disease were significant risk factors for acquisition of QRHI at our 500-bed hospital (New York Hospital Queens). All QRHI isolates were found to be genetically related by pulsed-field gel electrophoresis, were nontypeable, were susceptible to ceftriaxone and azithromycin, and were negative for β-lactamase production. Emphasis on patient contact and respiratory isolation and placing colonized or infected patients in cohorts yielded a marked reduction in the prevalence of QRHI at LTCF-A.