Immune profiling in human breast cancer using high-sensitivity detection and analysis techniques

ObjectivesEvaluation of immune profiles in human breast cancer using high-sensitivity detection and analysis methods.DesignCohort comparative analysis studies of breast tissue.SettingHuman hospital and laboratory healthcare facilities.ParticipantsWomen over 18 years.Main outcome measuresEvaluation of the comparative immunophenotype of human breast carcinoma and normal breast tissues.ResultsLeukocyte density and specific subgroups of lymphocytes and macrophages were generally higher in breast cancers compared to normal breast tissues. CD3, CD4, CD45RO, CD45RA(2H4), CD45 and HLA Class II (on TIL) were significantly expressed on breast tumour tissues compared with normal tissues (p < .01). Some 30% of T-cells were γδ-TCR positive, but the majority were αβ-TCR in type. CD19 (B-cell), CD14 (FMC32 and 33) and HLA Class I levels (epithelial and TIL) showed no significant differences. IL-2α receptor expression was low or absent on most TIL.ConclusionsHigh-sensitivity and image analysis techniques permitted accura...