Monitoring MHC Ubiquitination by MARCH Ubiquitin Ligases

: Ubiquitination is a reversible process that controls the intracellular transport of many transmembrane molecules. Ubiquitination of MHC I, MHC II, and CD1a by different members of the MARCH family of E3 ubiquitin ligases is a key event in the regulation of the potent immunostimulatory properties of activated dendritic cells. We describe here methods to monitor and quantify the ubiquitination levels of these different antigen presentation molecules and its impact on their cell surface accumulation.