Targeted and quantitative metabolomics in bacteria

Abstract Targeted metabolomics aims at reliable and sensitive quantification of a subset of metabolites in a cell. Here, we describe our current methods to obtain quantitative intracellular concentrations of a specified set of metabolites in bacteria. The protocol is structured into four steps of the workflow: standardized cultivation, sampling for metabolome analysis, extraction of metabolites and subsequent analysis by mass spectrometry. At the end, we give a brief discussion about the use of metabolite data.