Floresin İşaretleme ile Hücre Çeşitleme

In order to evaluate the effect of dexamethasone treatment upon the synthesis of DNA in human central nervous system cell cultures, nine different type cell cultures were used. Twin high cell density cultures for each cell type were used and one of these was treated with 10 ugr/ml dexamethasone for one week. These cells were investigated with FacS II (Beeton Dickinson) after treatment- with chromomycin A (Sigma lab). At the end of the research it was seen that, there were more cells in synthesis and myosis period in astrocytomas while there was partly blocage at G1 phase, which is related to the dexamethasone treatment, in metastatic and normal cells. insan SSS hucre kulturlerinde deksametazon tedavisinin DNA sentezi (S) uzerine etkisini saptamak amaciyla 9 degisik tip hucre kullanildi. Yuksek dansiteye ulasmis kulturlerden her hucre tipi icin bir kontrol ve bir de 1 haftalik 10 ugr/ml deksametazon tedavisi alan olmak uzere ikiser ornek hazirlandi. Bu hucreler chromomycin A (Sigma lab) ile muameleden sonra FacS II ile (Becton Dickinson) incelendi. Normal hucrelerle metastik tumor hucrelerinde deksametazonla tedaviye bagli Gi de kismi blok tesbit edilirken astrositik tumor hucrelerinde sentez ve mitoz devresine ulasmis hucre sayisi normal hucrelere oranla daha fazla bulundu.