Abstract 15486: Intramyocardial Gene Therapy With a Novel Angiogenic Molecule, Pellino-1 Preserves Functions and Increases Cell Survivability in a Mouse Myocardial Infarction Model

Introduction: Our present study attempted to evaluate the effect of Pellino1 (Peli1) overexpression on angiogenesis and myocardial function by intramyocardial administration of adenovirus encoding Peli1 (Ad.Peli1) following myocardial infarction (MI) in mice. Methods: CD-1/ICR mice were divided into six groups: Control Sham (CS); Control MI (CMI); Adeno-LacZ Sham (Ad.LacZS); Adeno-LacZ MI (Ad.LacZMI); Adeno-Pellino1 Sham (Ad.Peli1S) and Adeno-Pellino1 MI (Ad.Peli1MI). MI was induced by permanent ligation of left anterior descending coronary artery (LAD). Following LAD ligation or sham surgery Ad.LacZ or Ad.Peli1 was injected at 4 border zone sites to either Ad.LacZS/Ad.LaczMI or Ad.Peli1S/Ad.Peli1MI groups respectively. Heart tissue was collected 24 hours post MI for Western blot analysis,4 days for gap junction protein and 30 days later for immunohistochemistry after echocardiographic analysis. Results: Echocardiographic analysis revealed increased ejection fraction [48±0.75 vs. 33±0.98% (n=10-11);p<0.05...