Growth method for estimating the caloric availability of fats and oils

Several approaches are available for estimating the caloric availability of fats and fat substitutes. The method described here is a simple bioassay based on the growth of rats as a linear function of caloric intake from either a control fat such as corn oil or a fat of unknown caloric content. Excellent linear responses were found in the 14-day assay, and tests with cocoa butter, lard, and tallow showed agreement with previously reported values. The method is recommended as a simple method for estimation of the caloric value of fats