Purification of glycerophosphate oxidase isolated from mutant strain of aerococcus viridans

Crude extract isolated from Aerococcus viridans was purified by ethanol, ammonium sulphate or PEG precipitation followed by ion-exchange chromatography. The best purification (7.7-fold) and GPO recovery-81% were achieved when PEG precipitation as a first purification step was used. Gel FPLC following PEG precipitation yielded 92% of GPO but only 3.3-fold purification.