Expression of IGF1 protein in rats with cerebral ischemia and protective effect of Tongluojiunao injection.

Objective:To investigate the protective effects of IGF1 in rat cerebral ischemia brain by Tong luo jiu nao (TLJN) injection.Methods:using filament method to copy middle cerebral artery occlusion (MCAO) model of rats.We examined expression of IGF1 in brain tissue of rats undergone MCAO at 12 hours;24hours;3day and 7day by immunohistochemistry ,ELISA and RT-PCR and investigated TLJN protective effects correlated with the expression of NSE under ischemic condition by ELISA.Results:expression of IGF1 on rat brain increase after MCAO at 24h,and gradually decrease with time go by ,TLJN can increase IGF1 on rat brain and decrease the level of NSE.Conclusion:IGF1 had protective effects on rat cerebral ischemia brain injured by MCAO.TLJN can increase the expression of IGF1 in brain tissue to protect brain.