Evaluation of antimicrobial potential of Anemone obtusiloba D. Don.

Medicinal plants have been used from centuries to treat infectious diseases as an alternative form of health care. In recent years there has been intensifying attention in the detection of new antimicrobial compounds; due to alarming raise in the rate of diseases with multi-drug resistant microorganisms. In a search for plant extracts with antimicrobial activity the stem extract of Anemone obtusiloba was evaluated against Staphylococcus aureus and Escherichia coli by Agar disc-diffusion and micro dilution methods. The ethanolic (90%) extract showed significant effects with inhibition zones against S.ureus (10.6±0.5 mm) and E. coli (11.3±0.5 mm), with MIC’s against S.ureus (0.625 mg/ml) and E. coli (0.425 mg/ml). Tetracycline was used as standard drug for antimicrobial activity. The results demonstrate that the ethanolic (90%) extract of the stem of A. obtusiloba has significant antimicrobial activity and suggest that it may be useful in the treatment of infections.