Bioaccumulations and potential human health risks assessment of heavy metals in ppk-expressing transgenic rice

Abstract In order to reduce the phosphorus (P) resource consume, the polyphosphate kinase (ppk)-expressing transgenic rice (ETR) with high utilization efficiency of P fertilizer had been constructed. However, synthesis polyphosphates (polyP) mediated by ppk in the plants have the ability of chelating heavy metals, so the potential hazards of the new elite rice variety have raised concerns. In the study, we planted ETR and wild-type Nipponbare (WT) in paddy fields in southern China. After harvest, the concentrations of eight heavy metals in rice tissues were measured, and health risks assessments were performed. The field experiment showed that the ppk expressions were detected in the roots and straws of ETR plants but did not increase the concentrations of As, Cd, Cr, Ni and Pb in rice tissues. The Hg concentration in the ETRD root was 1.70-fold higher than that in WT, but the abundant Hg bioaccumulation in ETRD only occurred in the root. The bioaccumulation factors (BAFs) of all the detected heavy metals in the ETRS were no different from WT except for Cu and Zn. The results of human health risks assessment of heavy metals in brown rice showed that the non-carcinogenic risks of Cu or Zn in ETRD were higher than that in WT, while there was no difference in the total noncarcinogenic risk of the eight heavy metals in ETR. The carcinogenic risks of heavy metals in ETR were also comparable to that in WT. The results of this study indicated that the ppk expression in rice did not increase human health risks of heavy metals by consuming brown rice, which would provide a safety guarantee for agricultural and environmental applications of ETR not only with single-copy line but also with double-copy line.