A Simple and Rapid Method Based on Liquid Chromatography-Tandem Mass Spectrometry for the Measurement of α-L-Iduronidase Activity in Dried Blood Spots: An Application to Mucopolysaccharidosis I (Hurler) Screening

Jeong-Soo Yang,Hye Kyeong Min,Hyeon Ju Oh,Hye-In Woo,Soo Youn Lee,Jong-Won Kim,Junghan Song,Hyung-Doo Park

A Simple and Rapid Method Based on Liquid Chromatography-Tandem Mass Spectrometry for the Measurement of α-L-Iduronidase Activity in Dried Blood Spots: An Application to Mucopolysaccharidosis I (Hurler) Screening

2015

Jeong-Soo Yang
Hye Kyeong Min
Hyeon Ju Oh
Hye-In Woo
Soo Youn Lee
Jong-Won Kim
Junghan Song
Hyung-Doo Park

Background We developed an analytical method to measure α-L-iduronidase (IDUA) activity in dried blood spots. This was achieved by using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) with electrospray ionization in the positive ion mode.

Keywords:

Spots
Microbiology
Tandem mass spectrometry
Iduronidase
Liquid chromatography–mass spectrometry
Electrospray ionization
Mass spectrometry
Medicine
Pathology
Formic acid
Chromatography
Mucopolysaccharidosis I
Dried Blood Spot Testing
Triple quadrupole mass spectrometer
Sample preparation
Matrix (chemical analysis)

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