Twelve-color Chimerism Analysis after HLA-mismatched Hematopoietic Cell Transplantation Using Anti-HLA Antibody and Spectral Flow Cytometer

1. Abstract � Duringtheearlyphaseafterhematopoieticcelltransplantation� (HCT)�so-calledmixedchimerismsometimesoccursinperipheral� blood.�Donor/recipientchimerismanalysisusingtheHLA-Flowmethod� isaveryusefultechniqueforearlydiagnosisofgraftfailureandrelapse� ofleukemiaandforelucidationoftheunderlyingmechanismsinvarious� pathogenicstatesafterHCT.�Reportedhereinaretheresultsofseveral� twelve-colorchimerismanalysesusingtheSpectralflowcytometer� developedbySonyCorporation.�Theperformancewasequivalenttoa� commoncommercialinstrument.�Moreover,�oneoftheuniquefeatures� ofthisinstrumentistheabilitytodisplayeachflspectrum� foreachcell,�demonstratingtheaccuracyofanalysisandguarantying� furtheraccurateanalysisespeciallyinmulti-coloranalysis.