SNP Analysis of Prolactin Receptor Gene and Its Relationship with Semen Quality in Bulls

The exons and 5′UTR polymorphisms of PRLR gene in bulls were detected in this reserch by PCR-SSCP.The result showed that alleles A,B in exon 1 and C,D in exon 8 were detected in this population,among which A and C dominated,and there was no polymorphism in other exons and 5′UTR;The relationship between genetypes and performances was analyzed,and the results indicated that fresh ac integrity rate of genotype AA was higher than genotype AB in exon 1 and frozen semen energy of genotype CD was much higher than genotypes CC and CD in exon 8.It was initially concluded that PRLR is one of the candidate genes for breeding traits.