MP12-04 DETRUSOR OVERACTIVITY INDUCED BY CEREBRAL INFARCTION MAY BE ASSOCIATED WITH ENHANCED ACTIVITY OF RHO-KINASE IN THE BLADDER

bladder catheter through the dome. In the CMG measurements in a decerebrated condition, mice underwent cystostomy formation and supracollicular decerebration under isoflurane-anesthesia. After recovery from the anesthesia (waiting for at least 2 hours), CMG was carried out with saline-instillation at 10 ml/min. RESULTS: In the FV measurements, in females, mean voided volume and mean flow rate in TRPM2-KO mice were significantly greater than those in WT mice. In males, similar tendencies were observed, but there were no significant differences (n 1⁄4 10, in each group). In the conscious CMG measurements, in females, threshold pressure, micturition pressure, intercontraction interval and mean voided volume in TRPM2-KO mice were significantly greater than those in WT mice. In contrast, in male mice, only threshold pressure in TRPM2-KO mice was significantly higher than that in WT mice (Figure 1A, n 1⁄4 8 in each group). In the CMG measurements in the decerebrated condition, only threshold pressure in TRPM2-KO mice was significantly higher than that in WT mice in both sexes (Figure 1B, male TRPM2-KO n 1⁄4 7, other groups n 1⁄4 8). CONCLUSIONS: The present study suggests that TRPM2 channels may have a role in modulating bladder mechano-sensation in mice, and also that this contribution might be more remarkable in females.