Study on the influence of sodium nitroprusside on idiopathic thrombocytopenia purpura model mouse spleen antioxidants glutathione and oxidized glutathione content

Objective To investigate the impact of sodium nitroprusside (SNP) on the concentration of antioxidants glutathione(GSH) and oxidized glutathione (GSSG) in the spleens of idiopathic thrombocytopenia purpura (ITP).Method ITP model mice were divided into the nitric oxide (NO) donor drug SNP groups and phosphate buffered saline (PBS) control group.Intraperitoneal injection with the concentration (1 mg/ml) of SNP for SNP group while intraperitoneal injection using the same volume of PBS for PBS control group.The expression of NO,nitric oxide synthase (NOS) in peripheral blood and GSH / GSSG content in spleen was detected after medication for 5 min,15 min,25 min,35 min and 45min.Results Serum concentration of NO and NOS in SNP group were higher than the PBS control group in the first 5 min,15 min,25 min,35 min and 45min (NO:t5 =6.24,t15 =8.65,t25 =9.87,t35 =11.20,t45 =13.07;NOS:t5 =5.47,t15 =6.55,t25 =8.04,t35 =9.28,t45 =11.54,all P ＜ 0.05).NO and NOS content had statistical difference in each measurement time point (NO:t5-15 =3.04,t15-25 =4.11,t25-35 =3.82,t35-45 =3.54;NOS:t5-15 =3.98,t15-25 =5.04,t25-35 =5.12,t35-45 =4.26,all P ＜ 0.05).GSH concentration reduced with time while GSSG content increased with time (GSH:t5-15 =5.62,t15-25 =4.90,t25-35 =5.21,t35-45 =5.07;GSSG:t5-15 =4.07,t15-25 =6.57,t25-35 =4.98,t35-45 =5.41,all P ＜ 0.05).GSH concentrations were negatively correlated with NO,NOS (r =-0.625,r =-0.534,both P ＜ 0.01).GSSG concentrations were positively correlated with NO,NOS (r =0.714,r =0.687,both P ＜ 0.01).Conclusion NO donor drug SNP reducd the level of GSH in a time-dependent pattern in ITP mice,SNP may play a physiological function by affecting the system of antioxidant GSH / GSSG. Key words: Nitric oxide;  Nitric oxide synthase;  Glutathione;  Idiopathic thrombocytopenic purpura