Effect of ligustrazine on bone marrow hematopoiesis in mice after bone marrow transplantation

Objective: To investigate the effect of ligustrazine (LT) on hematopoiesis in mice after bone marrow isotransplantation (iso BMT). Methods: The typical model of iso BMT was established and the model mice were randomly divided into two groups, the LT group treated with LT injection 0.2 ml and the control group treated with normal saline 0.2 ml, twice a day by gastrogavage. The following parameters were observed in the day 1, 7 and 14: peripheral blood cells, bone marrow mono nuclear cells (BMMNC), heparin sulfate (HS) expression in bone marrow section by immunohistochemical SABC AP method, stromal cell derived factor 1 (SDF 1) expression and CXC chemotaxis factor receptor 4 (CXCR4) expression. Results: The levels of peripheral WBC, platelet, BMMNC, CXCR4, HS, SDF 1 at the day 7 and 14 in the LT group were all higher significantly than those in the control group (P0 05 or P0 01). Conclusion: LT could improve the bone marrow hematopoiesis in the early hematopoietic re establishing stage after BMT.