Flow cytometric evaluation of DNA stainability with propidium iodide after histone H1 extraction

A flow cytometric evaluation of the effect of the histone H1 extraction on DNA stainability with propidium iodide was performed on isolated HeLa nuclei. Selective removal of the lysine-rich protein was attained by using two established techniques involving treatment with 0.7 M NaCl or low pH. DNA stainability was monitored at different dye/DNA-P ratios, varying from low to high saturating concentrations. Depletion of the histone Hlfrom nuclei results in the transition from low to high affinity of a portion of binding sites, as shown by 1) the increase