Modulation of Antigenic Profile by Persistent Infection of Sendai Virus in Human Neuroblastoma

Summary Human neuroblastoma cells SK-N-MC were infected with murine parainfluenza type I Sendai Virus Two clones with persistent infection showed production of virus amplified in chicken embryos. One clone had a proliferation pattern similar to the uninfected cells, whilst the other clone showed reduced tritiated thymidine and uridine incorporation. Monoclonal antibodies UJ308, UJ13A, UJ127.11, UJ181.4, A2B5 were able to bind the cells; only αThy-1 antibody was negative. Moreover the binding of UJ308 was the only one positively modulated by Sendai infection These results show that SK-N-MC human neuroblastoma cells are a good “in vitro” model for studies on parainfluenza virus infection in the nervous system