Diagnostic value of combined detection of autoantibodies against p53 and Bmi-1 in lung cancer

Objective To evaluate the diagnostic value of combined detection of serum autoantibodies against against p53 and Bmi-1 in lung cancer (LC). Methods Serum levels of autoantibodies against p53 and Bmi-1 were measured by enzyme-linked immunosorbent assay (ELISA) in 92 patients with LC and 80 normal controls. The combined diagnostic value was evaluated with the receiver operating characteristic (ROC) curve. Results The serum levels of autoantibodies against p53 and Bmi-1 were significantly higher in LC than in normal controls (0.438±0.705 vs 0.045±0.035, Z=-7.667, P 0.05). The combined detection of two autoantibodies provided an enhanced sensitivity of 63.0%, a specificity of 91.2% and an area under curve (AUC) of 0.881, which showed better diagnostic efficiency compared to individual autoantibodies. Conclusions Combined detection of autoantibodies against p53 and Bmi-1 shows good diagnostic value, which may aid diagnosis of LC. Key words: Repressor proteins/DU; Nuclear proteins/DU; Tumor suppressor protein p53/DU; Autoantibodies/DU; Lung neoplasms/DI