Single-nuclei RNA-sequencing of plants
2020
Single-cell genomics has slowly advanced in plant research. Here, we introduce a generic procedure for plant nuclei isolation and nanowell-based library preparation for short-read sequencing. This plant-nuclei sequencing (PN-seq) method allows for analyzing several thousands of genes in thousands of individual plant cells. In this way, we expand the toolset for single-cell genomics in the field of plant biology to generate plant transcriptome atlases in development and environmental response.
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