The effect of single aerobic exercise on the regulation of mitochondrial biogenesis in skeletal muscles of trained men: A time-course study

The study focused on the effects of duration of a single aerobic exercise session of moderate intensity (60% of {ie296-1}) on the activation of PGC-1α gene expression-regulating signaling kinases and the expression of the genes that regulate mitochondrial biogenesis and play a role in regulating catabolism. Nine athletes ({ie296-2} 59 mL/min/kg) cycled on a ergometer for 30, 60, and 90 min. A exercise-induced increase in PGC-1α gene expression was found to occur without activation of the kinases AMPK, p38 MAPK, and CaMKII. A comparable increase in PGC-1α gene expression was observed after 60- and 90-min exercises, while VEGFA gene expression increased only after 90-min exercise. Even 90-min exercise of the given intensity did not activate the FOXO1-E3 ubiquitin ligase pathway, nor did it increase the expression of catabolism-regulating genes.