Markers of genotoxicity and oxidative stress in farmers exposed to pesticides

Abstract The effects of chronic exposure to pesticides can lead to the development of several diseases, including different types of cancer, since the genotoxic and mutagenic capacity of these substances can be observed. The objective of this study is to investigate the relation between the occupational exposure to various pesticides and the presence of DNA damage and oxidative stress. Blood samples from 50 rural workers (41 men and 9 women) exposed to pesticides, 46 controls (20 men and 26 women) from the same city (Antonio Carlos, Santa Catarina state, Brazil) and 29 controls (15 men and 14 women) from another city (Florianopolis, Santa Catarina state, Brazil), were evaluated using the comet assay and the cytokinesis-block micronucleus (CBMN) technique for genetic damage, and the test of thiobarbituric acid reactive substances (TBARS) and catalase (CAT) activity for the oxidative stress. Cholinesterase activities were also determined, but there was no statistical difference among exposed workers and controls. Significant differences were found in DNA damage among groups. The comet assay performed on peripheral blood lymphocytes of these individuals had a significantly higher DNA damage index in the exposed group comparing to controls (p