Fabrication and Characterization of Cobalt Hyaluronic acid Nanostructure via Gamma Irradiation for Improving Biomedical Applications

Abstract Aqueous dispersed cobalt hyaluronic acid nanostructure (CoHANs) were synthesized using cobalt ion (Co+2) as precursor and natural polysaccharide hyaluronic acid (HA) as stabilizing agent and gamma irradiation as reducing agent. The synthesized CoHANs are characterized by UV–Vis. spectroscopy, Dynamic light scattering (DLS), X-ray diffraction (XRD) and Fourier Transform Infrared spectroscopy (FT-IR). The morphology and surface appearance of CoHANs has been observed by SEM images. The particles size and shape of CoHANs were estimated by TEM images and was found to be 12.0 nm. XRD analysis of the CoHANs confirmed the formation of crystalline nanoparticles. The nucleation and growth mechanism of CoHANs is also discussed. The size of nanoparticles is influenced by certain parameters such as the choice of stabilizer and cobalt ion concentration and the absorbed dose. The results indicating the CoHANs possesses high activity than cobalt ion and HA. The present study explored the positive role of CoHANs as an antitumor agent on different cell carcinoma in vitro. Excellent bactericidal activity was shown by the CoHANs against pathogenic bacteria and fungi.