Protective effect of fasudil hydrochloride against acute renal injury in septicopyemia rats.

Abstract Objective To observe the protective effect of fasudil hydrochloride against acute renal injury in septicopyemia rats. Methods A total of 60 Wister rats were included in the study and divided into control group ( n  = 10), model group ( n  = 25) and treatment group ( n  = 25). Model group and treatment group received intraperitoneal injection of endotoxin (ET) to establish acute renal injury models while the control group only received daily intraperitoneal injection of normal saline 1 mL. Five rats were taken out of model group and treatment group respectively at 1 h (T1), 6 h (T2), 12 h (T3), 24 h (T4) and 48 h (T5), for intraperitoneal injection of ET 30 mg/kg. Treatment group received intraperitoneal injection of fasudil hydrochloride 30 mg/kg 1 h before injection of ET. For three groups, 5 mL blood samples were collected from postcava for determination of serum creatinine and urea nitrogen levels at different time points. Concentrations of serum tumor necrosis factor α and ET-1 were determined by using ELISA. The renal pathologic changes were observed under the microscope. Results Serum creatinine levels in both model group and treatment group were significantly higher than control group at T2–T5 ( P P P P P P P P Conclusions Fasudil hydrochloride has the significantly protective effect against acute renal injury in septicopyemia rats.