Effects of olive oil on oxidative stress in an experimental model of multiple sclerosis (P3.389)

Objective: The aim of the present work studies the effects of Extra-virgin olive oil (EVOO) administration in a myelin oligodendrocyte glycoprotein (MOG)-induced experimental autoimmune encephalomyelitis (EAE) model, laboratory equivalent to human multiple sclerosis (MS) Background: The data suggest that oxidative stress plays an important role in MS. In addition, epidemiological studies show that Mediterranean diet has neuroprotective effects. EVOO is the main constituent of Mediterranean diet. Design/Methods: Twenty male Dark Agouti rats, weighing 180–200g and 2 months old were used in the study. They were injected with an emulsion containing myelin oligodendrocyte glycoprotein (MOG, fragment 35–55) supplemented with heat-inactivated Mycobactirium tuberculosis H37Ra to reproduce experimental model of MS (experimental autoimmune encephalomyelitis, EAE). To study the protective effect of EVOO, the rats were supplemented with EVOO orally using catheter, representing 10% of calorie intake (in weight terms) in the total standard daily diet of rat. EVOO was applied during 51 days, starting 14 days after inoculation with MOG. The rats were divided into 4 groups of 5 as follow: i) control; ii) vehicle; iii) EAE; and iii) EAE+EVOO Results: Our data found that EAE prompted motor symptoms like paralysis and hind limbs, oxidative stress and weight loss compared with health animals. EVOO supplementation reduced motor symptoms, oxidative damage and weight loss. Additionally, EVOO caused the recovery of antioxidant syste Conclusions: In brief, these data indicated that: i) MOG induced an experimental model of MS; ii) EVOO administration shows positive and protective effects in EAE animals. Disclosure: E. Aguera Morales has received personal compensation for activities with Biogen, Sanofi Genzyme, and Novartis as a speaker and advisory board member. Dr. Conde-Gavilan has nothing to disclose. Dr. Luque-Carabot has nothing to disclose. Dr. LaTorre has nothing to disclose. Dr. Giraldo-Polo has nothing to disclose. Dr. Feijoo has nothing to disclose. Dr. Gascon-Luna has nothing to disclose. Dr. Lillo has nothing to disclose. Dr. Galvan-Jurado has nothing to disclose. Dr. Valverde-Moyano has received personal compensation for activities with Sanofi-Genzyme as a speaker. Dr. Escribano has nothing to disclose. Dr. Tunez-Finana has nothing to disclose.