Effect of the folic acid and vitamin B2 on the diabetes mellitus rats with diabetic nephropathy

Objective To explore the relationship among the folic acid,vitamin B 12 and diabetic nephropathy and to discuss the mechanism of the diabetic microangiopathy.Methods We selected 80 SD rats,and then divided them into 2 groups randomly.Those were the control group and the model groups.During the process of this study,the control group was fed with the normal animal feeds;the model groups were fed with the high calorie diets for 10 weeks.At the end of the tenth week,the rats of the model group were induced to diabetes mellitus by intraperitoneal injection with streptozocin 30mg/kg,and then we divided the diabetes mellitus rats into 4 groups which measured the levels of the blood fat(total cholesterin,triglyceride),Fins,FBG,SOD,MDA,GSH-Px.Results ①Through feeding the rats with the special feeds for a long time,and then injecting with streptozocin can make them develop to be the diabetes mellitus.The model groups'levels of the triglyceride,the cholesterin and the random blood glucose were(1.07±0.27),(2.29±0.42),(21.12±4.21) mmol/L,respectively,those were significantly higher than the control group's levels which were(1.11±0.20),(0.68±0.11),(5.73±0.26) mmol/L(P0.05).② After given different interference,for the group of the diabetes mellitus rats that without fed with vitaminB 12 and folic acid,the degree of the pathological change of kidney tissue was the most serious through the method of periodic acid-Schiff's staining(PAS),and the level of the MDA was also highest(P0.05),the level of the GSH-Px was lowest(P0.05) during the all groups.What's more,both of the folic acid and the vitamin B 12 could reduce the increase of the 24hours urinary albumin,especially using the folic acid and the vitamin B 12 together.Conclusion Using the special feeds to feed the SD rats for ten weeks and then inject STZ(30mg/kg) from abdominal cavity can make them develop to be the diabetes mellitus.The supplement with folic acid and vitamin B 12 had the effect of protecting the capillary of the kidney from damage,and the mechanism may have something to do with the effect of anti-oxygenation.