Fabrication of stable zein nanoparticles by chondroitin sulfate deposition based on antisolvent precipitation method

Abstract The purpose of this work is to stabilize zein nanoparticles with anionic polysaccharides—chondroitin sulfate (CS) to overcome the poor colloidal stability of zein nanoparticles. The average molecular weight of CS was 20.51 kDa and the disaccharide composition of CS was 70.71% monosulfated disaccharide Di2S and 29.29% disulfated disaccharide Di2,6 diS. Zein/CS composite nanoparticles were fabricated by antisolvent precipitation method at pH 4.0. The optimal mass ratio of zein to CS was 1:1 (w/w). Zein/CS composite nanoparticles showed mean size of 148 nm and PDI