Phytochemical Screening, Estimation of Total Phenol and Flavonoid Content of the Leaves of Smilax perfoliata Lour.

Plants are always part of traditional practice and have been a source of medicine since time immemorial. Based on such documents, researchers study their potential as medicine applying scientific knowledge. Such an effort was made to find out the possibility of biological activity through detecting the presence of phenols and flavonoids in the leaves of the plant Smilax perfoliata Lour. (Smilacaceae). The objective of the present study was phytochemical screening and quantitative estimation of total phenol and flavonoid contents in the leaf extracts of Smilax perfoliata Lour. using methnol and ethylacetate as menstrums. Standard biochemical and spectrophotometric methods were employed to study the phytochemical contents of the leaves of Smilax perfoliata Lour. Total phenol and flavonoid content of the extracts were spectrophotometrically determind. Phytochemical analysis of leaves of Smilax perfoliata Lour. showed the presence of Tannins, Flavonoids, Glycosides, Steroids, Alkaloids. Total phenol and flavonoid contents were found to be 38± 0.09 µgml -1 and 56 ± 0.24µgml -1 . Present study showed the presence of subtantial amount of phenolic and flavonoid components in the leaves of Smilax perfoliata Lour., which can be considered as good source for medicinal and food application.