Modulation of hyperthermia-induced platelet aggregation inhibition in the presence of urea.

AbstractPurpose: This study has been conducted to evaluate the effect of urea on aggregation responses of heat-treated platelets.Materials and methods: The urea was added to platelet-rich plasma (PRP) samples in final concentrations of 50 and 100 mM. PRP samples, with or without exogenous urea, were incubated at 37 °C, 39 °C and 41 °C for 90 min and then were stimulated with adenosine diphosphate (ADP) or epinephrine for measuring of platelet aggregation responses. The average reduction in aggregability of heat-treated samples with reference to mean value obtained for control samples treated at 37 °C was expressed as inhibition percentage.Results: Aggregation responses of the samples treated in the presence of 50 mM and 100 mM urea were significantly less inhibited by hyperthermia treatments compared with those treated without exogenous urea.Conclusion: The results indicate that the inhibitory effect of hyperthermia on platelet aggregation responses could be significantly modulated by urea.