Relationship of β 3 -adrenergic receptor polymorphism with metabolic syndrome and oxidative stress parameters in postmenopausal women

Introduction: Some studies indicate, that the Trp 64 /Arg 64 polymorphism of β 3 -adrenergic receptor (ADRB3) is associated with obesity, insulin resistance and earlier onset of type 2 diabetes mellitus.The aim of our study was evaluation of frequency of this ADRB3 polymorphism and his association with metabolic syndrome parameters and oxidative stress in postmenopausal women. Material and methods: We performed the study among 94 women, aged 50-60, selected randomly from Wroclaw city population. Estimation of anthropometric parameters, densitometry (total body fat, android and gynoid deposits - using DPX(+) Lunar, USA device) and biochemical estimations such as lipid profile, glucose, insulin, estradiol and FSH serum level (using commercial kits) were carried out. Oxidative stress was estimated by measurement of thiobarbituric-reactive substances (TBARS) serum concentration, using Yagi method, on spectrofluorimeter Perkin-Elmer LS55. Blood for analysis was collected before, direct after and 6 h after the 30-minutes physical test using cycloergometer. ADRB3 genotyping was performed by PCR and minisequencing using ABI 310 sequencer (Applied Biosystems). Results: The frequency of Trp 64 /Arg 64 genotype in investigated population was 15.8%. The Arg 64 /Arg 64 genotype had only one woman. Women bearing Trp 64 /Arg 64 genotype showed higher mean serum level of triglycerides and lower serum level of HDL-cholesterol in comparison to women bearing Trp 64 /Trp 64 genotype, however without statistical significance (p > 0.05) (respectively, means ± SD for triglycerides: 140.3 ± 64.1 vs. 113.9 ± 56.2 mg/dl; and for HDL-cholesterol: 60.9 ± 11.9 vs. 67.0 ± 16.9 mg/dl). Both groups did not differ in any other investigated anthropometric nor biochemic parameter. Conclusions: 1. The Trp 64 /Arg 64 polymorphism of β 3 -adrenergic receptor could be associated with lipid profile disoders observed in metabolic syndrome in postmenopausal women, however it should be explaned basing on the study with more included subjects. 2. The Trp 64 /Arg 64 polymorphism of β 3 -adrenergic receptor has no influence on oxidative stress intensification after standardized physical effort in postmenopausal women. (Pol J Endocrinol 2007; 58 (3): 201-206)