The effect and mechanism investigation of intermedin on the apoptosis of bone marrow derived mesenchymal stem cells induced by hydrogen peroxide

Objective To investigate the effect and mechanism of intermedin (IMD)on the apoptosis of BMSCs induced by H2O2 and its mechanism. Methods BMSCs being cultured in vitro. After 30 min pretreatment with 10-6mol/L IMD, cells were cultured in presence of 100 μmol/L H2O2 for 2 hour, cell apoptosis was determined by flow cytometry; Apoptosis related protein expression was determined by Western blotting and immunofluorescence; Protein and phosphorylation of ERK1/2 was detected by Western blotting and observe the influence of IMD after pretreatment with inhibitor of ERK1/2. Results IMD could increase phosphorylation of ERK1/2 pathway of BMSCs (P<0.01). Pretreatment of IMD could decrease BMSCs apoptosis induced by H2O2, and up regulation expression of Caspase-3 and Bax, down regulation expression of Bcl-2 (P<0.01), and the effect was abrogated by U0126. Conclusion IMD can activate ERK1/2 signaling pathway of BMSCs, and can regulate the expression of apoptosis related proteins. Furthermore, it can protect BMSCs from apoptosis induced by H2O2. Activation of ERK1/2 pathway was involved in the effect of IMD on apoptosis. Key words: Intermedin; Bone marrow mesenchymal stem cells; Apoptosis; Apoptosis related proteins