Hepatoprotective effect of Fufang-Huanglu oral liquid on α- naphthylisothiocyanate-induced hepatitis jaundice in mice

Purpose : To investigate the effect of Fufang-Huanglu Oral Liquid (HOL) on hepatitis jaundice in mice. Methods : A total of 72 mice were divided into 6 groups (n = 12): normal group,  control group (model group), positive-treated group, and 3 HOL treatment groups (7.5, 15 and 30 mL/kg). Mice in normal and control groups received normal saline (20 mL/kg) orally, while positive and HOL-treated mice were orally administered Huganning tablets (1.0 g/kg) and HOL (7.5, 15 and 30 mL/kg), respectively. After 8 days, all mice (except normal group) were orally administered  α-naphthylisothiocyanate (ANIT, 100 mg/kg) to induce hepatitis jaundice, and sacrificed 2 days after drug administration. Serum GPT, GOT and TNF-α, as well as liver index, MDA, SOD and lipid profiles were determined. Results : The results showed that HOL, at all doses, significantly decreased liver index, serum GPT, serum SGOT and serum  TNF-α (p < 0.01). HOL also significantly decreased MDA, total cholesterol, TC and triglycerides, TG (p < 0.01), but increased  liver SOD (p < 0.01). Histological results indicate that HOL ameliorated liver injury induced by ANIT. Conclusion : These results showed that HOL possesses significant hepatoprotective effects against liver injury. Keywords : Fufang-Huanglu Oral Liquid, Hepatoprotective, Mice, Hepatitis, Jaundice, α-Naphthylisothiocyanate, Liver index