Layer-by-layer construction of lipid bilayer on mesoporous silica nanoparticle to improve its water suspensibility and hemocompatibility

Mesoporous silica nanoparticle was expected to provide a versatile drug delivery platform with modification flexibility. To improve its hemocompatibility and realize controlled and/or targeting drug release, modification of bare mesoporous silica nanoparticles is essential. Herein, a novel method of coating mesoporous silica nanoparticles with lipid bilayers was developed. First, a homemade organosiloxane precursor was used for hydrophobic modification of mesoporous silica nanoparticles, then phospholipids (1,2-dihexadecanoyl-sn-glycero-3-phosphocholine and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)]) were coated by a filming-rehydration method based on hydrophobic interaction. The nanoparticle samples were characterized by transmission electron microscope, dynamic light scattering, nitrogen sorption, Fourier transform infrared, and thermal gravimetric analysis. Furthermore, the novel lipid bilayer coated mesoporous silica nanoparticles were compared with bare mesoporous silica nanoparticles in terms of suspension stability, drug release, hemolysis, and nonspecific protein absorption. Our data proved that lipid bilayer coated mesoporous silica nanoparticles had better hemobiocompatibility and controlled drug release properties than that of bare mesoporous silica nanoparticles.