Establishment and evaluation of combining disease and syndrome model of asthma with abnormal savda syndrome

Objective: To establish and evaluate a model combining disease and syndrome of asthma with abnormal savda syndrome.Methods: Multiple factors were used to establish abnormal savda rat model.Then,rats were sensitized and challenged by ovalbumin and further model combining disease and syndrome of asthma with abnormal savda syndrome was established.General state,behavior,hypothalamic-pituitary-adrenal axis function,sympathetic/parasympathetic function,airway hyperresponsiveness,inflammation,collage deposition were observed.Results: Weight loss(P0.05),increased diet and water(P0.05),diminished activities in OFT test(P0.05),decreased preference for sugar(P0.05) were significantly appeared in the abnormal savda group and combining asthma with syndrome group,which was similar to the abnormal savda clinical manifestations.Furthermore,the level of CORT and ACTH in plasma were increased(P0.05),but cAMP,cAMP/cGMP were decreased(P0.05).Airway resistance was remarkably increased(P0.05) and the balance of Th1/Th2 was disturbed,which Th2 cytokines(P0.05) were dominated in the asthma group and combining disease with syndrome group.HE staining showed that inflammatory cells infiltration in the lungs(P0.05);PAS staining demonstrated mucus overproduction(P0.05) and goblet cell hyperplasia(P0.05);MASSON staining indicated emerge of collagen deposition(P0.05) in the OVA-exposed groups.Moreover,the combining disease with syndrome group was more severe than the asthma group.Conclusion: Usage of multiple factors and ovalbumin exposure could successfully establish combining disease and syndrome model of asthma with abnormal savda syndrome,and this model had either the syndrome performance of abnormal savda in Uygur medicine,or the characteristic of pathology of asthma such as airway hyperresponsiveness,airway inflammation and airway remodeling,which indicated the feasibility of this model.