Self-polymerized Dopamine Decorated Au NPs and Coordinated with Fe-MOF as a Dual Binding Sites and Dual Signal-amplifying Electrochemical Aptasensor for the Detection of CEA

Fabrication of functional electrochemical biosensor is a hot topic, however, precise and sensitive cancer detection in early clinical diagnosis is still a great challenge. Continuous efforts has been devoted to explore functional materials for this issue. In this work, we developed a dual binding sites and dual signal-amplifying electrochemical aptasensor of self-polymerized dopamine decorated Au and coordinated with Fe-MOF (Au@PDA@Fe-MOF) for the detection of carcinoembryonic antigen (CEA). Remarkably, Au@PDA@Fe-MOF features high sensitivity, multiple active sites, good biocompatibility and excellent selectivity which is attributed to abundant -COOH in porous Fe-MOF and unsaturated Fe3+ sites on the surface of Fe-MOF as the active binding sites grafting more NH2-functionalized CEA-specific aptamer and redox PDA and Fe-MOF accelerating the movement of electrons for dual signal amplifying. Meanwhile, the electrochemical aptasensor shows favorable repeatability with 1.82% Relative Standard Deviation (RSD) u...