Genetic characteristics of enterovirus 71 Hangzhou isolate H3-TY

Objective To investigate genetic characteristics of enterovirus 71(EV71) Hangzhou isolate H3TY and explore the possibility of vaccine development.Methods The whole genome of EV71 strain H3-TY was amplified by RT-PCR and cloned into plasmid for sequencing.The genome sequence was thus obtained by splicing sequences from individual clones and was taken homology and phylogenetic analysis with C4,TW and BrCr strains by Lasergene and MEGA.Results The strain H3-TY belonged to subtype C4.When compared to other domestic strains,the homology value of nucleotide was between 94.0％ and 99.0％,and the homology value of amino acid was over 97％.H3-TY major antigen site SP70 was the same as other strains,and SP55 was also the same as other strains,but slightly different fiom the BJ08 strain and BrCr strain.Conclusions EV71 strain H3-TY has a high level of homology with other domestic strains and shares the same major antigen site,which could be potentially useful for development of EV71 vaccine. Key words: Sequence homology;  Enterovirus 71;  Sequence analysis