Study on micro column gel technique application to pretransfusion tests in ABO hemolytic diseases of newborn

Objective:To investigate the optimal protocol of pretransfusion tests and the transfusion policy for neonates.Method:20 samples of serum and elution from RBC of neonates with ABO-HDN were collected and crossmatched with 5 random RBC samples from RBC components simultaneously by manual polybrene test and micro column gel technique,then the difference between the two crossmatching methods and their relationship with direct antiglobulin test of ABO-HDN were assessed.Result:①Among 100 times of crossmatching tests,the incompatible numbers from serum and elution with micro column gel technique were 74 and 62,those numbers with manual polybrene test were 3 and 5.No one case with solely incompatible result from manual polybrene test accompanied by compatible from micro column gel technique was occurred.②Apart from incompatible crossmatching results in serum with manual polybrene test,the incompatible frequency of serum and elution in ABO-HDN neonates with positive direct antiglobulin test was high than those with negative direct antiglobulin test(P0.05).③There had no significant difference in the agreement of incompatible results from serum(Kappa=0.021,P=0.297) and elution(Kappa=0.063,P=0.072) by the two crossmatching methods,the sensitivity of manual polybrene test was obviously lower in crossmatching test.Conclusion:Direct antiglobulin test is essential for pretransfusion tests in neonates,and micro column gel technique is the more appropriate method for the crossmatching test in neonates.The best transfusion policy in neonates was the selection of blood components with the identical or compatible blood group by the diagnosis of hemolytic diseases of newborn in advance.