The potential of small molecules to modulate glycosylation by media design.

Background A large number of recent publications demonstrate the effect of cell culture media on post-translational modifications of recombinant proteins [1]. In this study, aiming to extend the toolbox of media design beyond the commonly known media components, a large variety of cell culture compatible chemical components such as sugars in industrial relevant Chinese hamster ovary cell lines (CHO) expressing recombinant antibodies were identified and tested. One promising novel supplement is presented hereafter.