Effects of two organic pollutants on biomarker system of fish Lateolabrax japonicus and the pollution assessment

Responses of a select suite of protective enzymes in Lateolabrax japonicus including superoxide dismutase(SOD),catalase(CAT),glutathione peroxidase(Gpx),reduced glutathione(GSH) glutathione S-transferase(GST),nitric oxide synthase(iNOS) in liver tissue and brain acetylcholinesterase(AChE),which could work as sensitively biochemical biomarkers were analyzed when exposed to different concentrations of sodium dodecylbenzene sulfonate(SDBS) and benzo[a]pyrene(B[a]P) under controlled laboratory conditions.Results showed that:① The biochemical biomarkers included in this investigation,different responses occurred in the enzymatic activities when exposed to different pollutants:the activities of SOD,GST,Gpx and GSH content were significantly induced by B[a]P(p0.05) while others present little different as compared to the control.Differently,SDBS exposure greatly affected the activities of CAT,Gpx,iNOS and AChE while showed little effect on the other evaluated biomarkers.Gpx was the most sensitive biomarker that sensitively indicated the organic pollution stress.② The simultaneous assay on heat shock protein 70(Hsp70) expression was conducted by flow cytometry(FCM) and result presented that Hsp 70 expression could be effectively induced by SDBS as compared to the control throughout the experiment;however,B[a]P obviously induced Hsp70 during the first 12 d and then decreased to the level of the control.③ The evaluated method of biomarker system combined with principal component analysis (PCA) could effectively distinguish different pollutants under controlled laboratory conditions and might be a promising and warning method for the early assessment of environmental change exposed to different organic pollutions.