Production and refining method of gardenia red pigment

2013 
The present invention discloses a method for the production and purification of gardenia red pigment, is in the field of natural pigments. The method of the present invention comprises the steps of: gardenia yellow aqueous extract was macroporous resin equilibration, the column with deionized water and 20% ethanol solution to give Geniposide; Geniposide after alcohol was added to the mixture was concentrated alkaline hydrolysis reaction of sodium hydroxide and then acidified with citric acid was added; and the sodium glutamate geniposide pretreated fermentation broth from Aspergillus niger was added, the reaction incubated gardenia red pigment obtained conversion solution; gardenia red conversion It was treated with enzyme inactivation boiling water bath, allowed to cool, centrifuged and the supernatant stand; acid precipitation method or resin transfer method gardenia red pigment off after the enzymatic conversion of purified supernatant obtained Gardenia red pigment powder. The method of the present invention each unit operation are very simple, low energy consumption and manpower, the entire production process can be continuous, industrial production of high efficiency, economical and environmentally friendly, the product bright color and high purity.
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