188 The Influence of Maternal Obesity on Perivascular Adipose Tissue Function in Offspring

Objective Maternal obesity pre-programmes offspring to develop obesity, glucose intolerance and associated cardiovascular disease later in life although the underlying mechanism is currently unknown. This study investigated the effect of a maternal high fat diet on perivascular adipose tissue (PVAT) regulation of resistance artery tone. Design and method 8 week old female SD rats were fed a 10% fat diet (controls) or 45% fat obesogenic diet (HFD) for 12 weeks before mating then continued on their respective diets during pregnancy and lactation. PVAT-intact or -denuded mesenteric arteries from dams and pups (250–300 µm internal diameter) were mounted on a wire myograph. Cumulative concentration-response curves were constructed to thromboxane A 2 receptor agonist U46619 (10 nM–3 µM) ± 10 µM A769662, an activator of AMP-activated kinase (AMPK), and/or 100 µM L-NMMA, a nitric oxide synthase (NOS) inhibitor. Results Body weight (BW) and systolic (SBP) and diastolic (DBP) blood pressure were significantly increased in HFD dams (BW: p Conclusions In summary, the attenuated anti-contractile effects of PVAT in HFD dams and their offspring may be modulated by AMPK; however it is not totally dependent on nitric oxide release.